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flow cytometer attune nxt  (Thermo Fisher)


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    Structured Review

    Thermo Fisher flow cytometer attune nxt
    Flow Cytometer Attune Nxt, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/attune+nxt+flow+cytometer/attune+nxt+flow+cytometer/pmc12226097-136-42-47
    Average 90 stars, based on 1 article reviews
    flow cytometer attune nxt - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Flow Cytometry:

    Article Title: Activation of the aryl hydrocarbon receptor with structurally diverse ligands suppresses class switch to immunoglobulin A.
    Article Snippet: At the termination of culture, cells were stained with PE-conjugated anti-IgA (Invitrogen, 12-4204-81), PE anti-IgG2b (BioLegend; 406707), or the matched isotype control, and Sytox Blue (ThermoFisher, L34958) for viable cell identification. .. A minimum of 10,000 cells were analyzed per sample using an Attune NxT flow cytometer (Thermo-Fisher). ..

    Article Title: Amphiphilic albumin-based nanoparticles designed for the efficient delivery of taxanes.
    Article Snippet: .. The Attune NxT Flow Cytometer (Thermo Fisher Scientific, UK) was used to measure the fluorescent intensity of the fluorescein isothiocyanate (FITC, #F7250, Sigma Aldrich, UK) labelled samples in cells. ..

    Article Title: Neuraminidase delivered as an APC-targeted DNA vaccine induces protective antibodies against influenza
    Article Snippet: Bound vaccine proteins were detected with 2 mg/mL biotinylated anti-A/B followed by 2 mg/mL streptavidin-PE (BioLegend). .. Cells were fixed with 2% paraformaldehyde and analyzed using an Attune NxT flow cytometer (Thermo Fisher Scientific) and FlowJo software v.10.6.1 (BD Biosciences). www.moleculartherapy.org In vivo DNA vaccination of BALB/c mice DNA vaccine plasmids were prepared with Endofree Mega Prep Kits (QIAGEN) and dissolved in 0.9%NaCl (B. Braun). .. BALB/cmice were anesthetized i.p. with 0.2 mL tiletamine/zolazepammix (Zoletil Forte, 250 mg/mL, Virbac), xylazine (Rompun, 20 mg/mL, Bayer Animal Health), and fentanyl (50 mg/mL, Actavis).

    Article Title: Envelope protein-specific B cell receptors direct lentiviral vector tropism invivo
    Article Snippet: For bioluminescence imaging and flow cytometry analysis of transduced splenic cells, lentiviral vectors expressing LumiScarlet (FUFlagLumiScltW) pseudotyped with the VSV-G, Sindbis, or 2.2 1LFLAG1L envelope protein were retroorbitally injected into C57BL6 albino mice (female, 6 weeks old; The Jackson Laboratory, Bar Harbor, ME, USA). .. Flow cytometry analyses were performed on an Attune NxT flow cytometer (Thermo Fisher Scientific), and data were analyzed by FCS Express 5 (De Novo Software, Pasadena, CA, USA). .. Four days post vector administration, we anesthetized the mice and intraperitoneally injected them with fluorofurimazine (8.8 mM 100 mL/mouse; Promega, Madison, WI, USA).

    Article Title: Supramolecular assembly of polycation/mRNA nanoparticles and in vivo monocyte programming
    Article Snippet: The antibodies used in the experiments included anti-CD45 BV421 (BioLegend, Cat. #103134), anti-CD11b AF488 (BioLegend, Cat. #101217), anti-CD11b BV785 (BioLegend, Cat. #101243), anti-F4/80 PerCP-Cy5.5 (BioLegend, Cat. #123128), anti-F4/80 BV785 (BioLegend, Cat. #123141), anti-CD11c APC (BioLegend, Cat. #117310), anti-CD11c AF700 (BioLegend, Cat. #117320), anti-Ly6G APC-Cy7 (BioLegend, Cat. #127624), anti-Ly6C APC (BioLegend, Cat. #128016), antiCD3 AF488 (BioLegend, Cat. #100210), anti-CD19 APC (BioLegend Cat. #115512), anti-CD326 PerCP-Cy5.5 (BioLegend Cat. #118220) and anti-CD31 AF700 (BioLegend, Cat. #102444). .. Stained cells were analyzed on an Attune NxT Flow Cytometer (Thermo Fisher Scientific), and data processed by FlowJo (version 10.8.1, BD). ..

    Article Title:
    Article Snippet: EdU was labeled with Alexa fluor 647-azide (Life Technology) by incubating cells in EdU labeling solution (1 mM CuSO4, 1 μM AF-647, 100 mM ascorbic acid, diluted in PBS) for 30 minutes at room temperature in the dark. .. Cells were analyzed on an Attune NxT flow cytometer (Thermo Fisher Scientific) and the results were analyzed using FCS Express 7 Research (De Novo Software). .. DNA content was labeled by incubating cells in 1 μg/mL DAPI (Sigma-Aldrich Chemistry) supplemented with 100 μg/mL RNase (Sigma-Aldrich Chemistry) (diluted in PBS + 1% BSA + 0.5% Triton X-100) for either 1 hour at 37°C or overnight at 4°C (in the dark).

    Cytometry:

    Article Title: Amphiphilic albumin-based nanoparticles designed for the efficient delivery of taxanes.
    Article Snippet: .. The Attune NxT Flow Cytometer (Thermo Fisher Scientific, UK) was used to measure the fluorescent intensity of the fluorescein isothiocyanate (FITC, #F7250, Sigma Aldrich, UK) labelled samples in cells. ..

    Article Title: Supramolecular assembly of polycation/mRNA nanoparticles and in vivo monocyte programming
    Article Snippet: The antibodies used in the experiments included anti-CD45 BV421 (BioLegend, Cat. #103134), anti-CD11b AF488 (BioLegend, Cat. #101217), anti-CD11b BV785 (BioLegend, Cat. #101243), anti-F4/80 PerCP-Cy5.5 (BioLegend, Cat. #123128), anti-F4/80 BV785 (BioLegend, Cat. #123141), anti-CD11c APC (BioLegend, Cat. #117310), anti-CD11c AF700 (BioLegend, Cat. #117320), anti-Ly6G APC-Cy7 (BioLegend, Cat. #127624), anti-Ly6C APC (BioLegend, Cat. #128016), antiCD3 AF488 (BioLegend, Cat. #100210), anti-CD19 APC (BioLegend Cat. #115512), anti-CD326 PerCP-Cy5.5 (BioLegend Cat. #118220) and anti-CD31 AF700 (BioLegend, Cat. #102444). .. Stained cells were analyzed on an Attune NxT Flow Cytometer (Thermo Fisher Scientific), and data processed by FlowJo (version 10.8.1, BD). ..

    Software:

    Article Title: Neuraminidase delivered as an APC-targeted DNA vaccine induces protective antibodies against influenza
    Article Snippet: Bound vaccine proteins were detected with 2 mg/mL biotinylated anti-A/B followed by 2 mg/mL streptavidin-PE (BioLegend). .. Cells were fixed with 2% paraformaldehyde and analyzed using an Attune NxT flow cytometer (Thermo Fisher Scientific) and FlowJo software v.10.6.1 (BD Biosciences). www.moleculartherapy.org In vivo DNA vaccination of BALB/c mice DNA vaccine plasmids were prepared with Endofree Mega Prep Kits (QIAGEN) and dissolved in 0.9%NaCl (B. Braun). .. BALB/cmice were anesthetized i.p. with 0.2 mL tiletamine/zolazepammix (Zoletil Forte, 250 mg/mL, Virbac), xylazine (Rompun, 20 mg/mL, Bayer Animal Health), and fentanyl (50 mg/mL, Actavis).

    Article Title: Envelope protein-specific B cell receptors direct lentiviral vector tropism invivo
    Article Snippet: For bioluminescence imaging and flow cytometry analysis of transduced splenic cells, lentiviral vectors expressing LumiScarlet (FUFlagLumiScltW) pseudotyped with the VSV-G, Sindbis, or 2.2 1LFLAG1L envelope protein were retroorbitally injected into C57BL6 albino mice (female, 6 weeks old; The Jackson Laboratory, Bar Harbor, ME, USA). .. Flow cytometry analyses were performed on an Attune NxT flow cytometer (Thermo Fisher Scientific), and data were analyzed by FCS Express 5 (De Novo Software, Pasadena, CA, USA). .. Four days post vector administration, we anesthetized the mice and intraperitoneally injected them with fluorofurimazine (8.8 mM 100 mL/mouse; Promega, Madison, WI, USA).

    Article Title:
    Article Snippet: EdU was labeled with Alexa fluor 647-azide (Life Technology) by incubating cells in EdU labeling solution (1 mM CuSO4, 1 μM AF-647, 100 mM ascorbic acid, diluted in PBS) for 30 minutes at room temperature in the dark. .. Cells were analyzed on an Attune NxT flow cytometer (Thermo Fisher Scientific) and the results were analyzed using FCS Express 7 Research (De Novo Software). .. DNA content was labeled by incubating cells in 1 μg/mL DAPI (Sigma-Aldrich Chemistry) supplemented with 100 μg/mL RNase (Sigma-Aldrich Chemistry) (diluted in PBS + 1% BSA + 0.5% Triton X-100) for either 1 hour at 37°C or overnight at 4°C (in the dark).

    In Vivo:

    Article Title: Neuraminidase delivered as an APC-targeted DNA vaccine induces protective antibodies against influenza
    Article Snippet: Bound vaccine proteins were detected with 2 mg/mL biotinylated anti-A/B followed by 2 mg/mL streptavidin-PE (BioLegend). .. Cells were fixed with 2% paraformaldehyde and analyzed using an Attune NxT flow cytometer (Thermo Fisher Scientific) and FlowJo software v.10.6.1 (BD Biosciences). www.moleculartherapy.org In vivo DNA vaccination of BALB/c mice DNA vaccine plasmids were prepared with Endofree Mega Prep Kits (QIAGEN) and dissolved in 0.9%NaCl (B. Braun). .. BALB/cmice were anesthetized i.p. with 0.2 mL tiletamine/zolazepammix (Zoletil Forte, 250 mg/mL, Virbac), xylazine (Rompun, 20 mg/mL, Bayer Animal Health), and fentanyl (50 mg/mL, Actavis).

    other:

    Article Title: Materials and Methods
    Article Snippet: After incubation, cells were washed once more and resuspended in PBSF for cell sorting (Attune NxT Flow Cytometer, Thermo Fisher Scientific).

    Article Title: Exploring CDK4/6-Dependencies in ex vivo Ovarian Cancer Models
    Article Snippet: Cells were filtered, analysed on an Attune TM NxT flow cytometer (ThermoFisher) with 30,000 events collected per sample using Attune NxT Software.

    Staining:

    Article Title: Supramolecular assembly of polycation/mRNA nanoparticles and in vivo monocyte programming
    Article Snippet: The antibodies used in the experiments included anti-CD45 BV421 (BioLegend, Cat. #103134), anti-CD11b AF488 (BioLegend, Cat. #101217), anti-CD11b BV785 (BioLegend, Cat. #101243), anti-F4/80 PerCP-Cy5.5 (BioLegend, Cat. #123128), anti-F4/80 BV785 (BioLegend, Cat. #123141), anti-CD11c APC (BioLegend, Cat. #117310), anti-CD11c AF700 (BioLegend, Cat. #117320), anti-Ly6G APC-Cy7 (BioLegend, Cat. #127624), anti-Ly6C APC (BioLegend, Cat. #128016), antiCD3 AF488 (BioLegend, Cat. #100210), anti-CD19 APC (BioLegend Cat. #115512), anti-CD326 PerCP-Cy5.5 (BioLegend Cat. #118220) and anti-CD31 AF700 (BioLegend, Cat. #102444). .. Stained cells were analyzed on an Attune NxT Flow Cytometer (Thermo Fisher Scientific), and data processed by FlowJo (version 10.8.1, BD). ..



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